Increasing neuronal glucose uptake attenuates brain aging and promotes life span under dietary restriction in Drosophila

Summary Brain neurons play a central role in organismal aging, but there is conflicting evidence about the of neuronal glucose availability because uptake and metabolism are associated with both aging extended life span. Here, we analyzed metabolic changes brain Drosophila during aging. Using genetically encoded fluorescent adenosine triphosphate (ATP) biosensor, found decreased ATP concentration somata aged flies, correlated content, expression transporter glycolytic enzymes mitochondrial quality. The age-associated reduction did not occur suppressed glycolysis or enhanced uptake, suggesting these pathways contribute to reductions. Despite damage, increasing maintained levels, locomotor deficits, Increasing dietary restriction resulted longest spans, an additive effect enhancing bioenergetic challenge on